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Course Outline
Introduction to NGS and Forensic Applications
- Overview of DNA sequencing technologies
- Benefits of NGS compared to traditional forensic methods
- Key forensic applications of NGS
Fundamentals of Forensic Genetics
- DNA markers utilized in forensic analysis
- Short Tandem Repeats (STRs) and Single Nucleotide Polymorphisms (SNPs)
- Mitochondrial DNA and Y-chromosome analysis
NGS Workflows in Forensic Casework
- Sample preparation and library construction
- Sequencing protocols tailored for forensic samples
- Quality control measures and contamination prevention
Bioinformatics and NGS Data Analysis
- Processing and alignment of NGS data
- Variant calling and forensic interpretation
- Software tools for forensic genomic analysis
Forensic Anthropology and Ancestry Determination
- Genetic markers for ancestry and population studies
- NGS applications in analyzing ancient and degraded DNA
- Case studies in forensic anthropology
NGS for Challenging and Degraded Samples
- Techniques for low-quality and low-quantity DNA
- Managing environmental and mixed DNA samples
- NGS roles in missing persons and disaster victim identification
Legal, Ethical, and Regulatory Considerations
- Privacy issues in forensic genomics
- Regulatory frameworks for forensic DNA databases
- Ethical challenges in forensic genetic analysis
Future Trends in Forensic Genomics
- Advancements in sequencing technology
- Integration of AI in forensic genetics
- Emerging forensic applications of NGS
Summary and Next Steps
Requirements
- Fundamental knowledge of genetics and forensic science
- Familiarity with DNA sequencing concepts
Audience
- Forensic scientists and geneticists
- Anthropologists and bioarchaeologists
- Law enforcement and forensic investigators
14 Hours
